Monocytes of Patients Congenitally Deficient in Plasma Factor XIII Lack Factor XIII Subunit A Antigen and Transglutaminase Activity

Summary

Monocytes isolated from patients with severe deficiency in plasma Factor XIII of blood coagulation (FXIII) were tested for FXIII antigen and transglutaminase activity. By immunoperoxi- dase method the patients’ monocytes, in contrast to normal controls, showed no reaction with a monospecific antibody against FXIII subunit ª.This result was confirmed by immuno- blotting technique, as well. In addition, tissue macrophages tested in one of the patients were also exempt of FXIII subunit ª antigen. The transglutaminase activity in FXIII deficient monocytes was below the limit of the detection of the dansylcadaverine incorporation assay. The results suggest that FXIII subunitªof monocytes/macrophages and its plasma and platelet counterparts are closely related or identical proteins and demonstrate that the transglutaminase activity in monocytes is of FXIII origin and tissue transglutaminase is present, if at all, only in insignificant amount.

Keywords

Factor XIII - Monocytes - Transglutaminase

• References

• 1 Lorand L, Losowsky MS, Miloszewski KJ M. Human factor XIII: fibrin-stabilizing factor. Progr Haemostas Thrombos 1980; 5: 245-290
• 2 McDonagh J. Structure and function of Factor XIII. In: Haemostasis and Thrombosis. Colman RW, Hirsh J, Marder VJ, Salzman EW. (eds) pp 164-173 Lippincott; Philadelphia: 1982
• 3 Muszbek L, Laki K. Interaction of thrombin with proteins other than fibrinogen (thrombin susceptible bonds). Activation of FXIII. In: The Thrombin Machovich R. (ed) pp 321-342 CRC Press; Boca Raton: 1984
• 4 Muszbek L, Adany R, Szegedi G, Polgar J, Kavai M. FXIII of blood coagulation in human monocytes. Thrombos Res 1985; 37: 401-410
• 5 Adany R, Belkin A, Vasilevskaya T, Muszbek L. Identification of blood coagulation factor XIII in human peritoneal macrophages. Eur J Cell Biol 1985; 38: 171-173
• 6 Henriksson P, Becker S, Lynch G, McDonagh J. Identification of intracellular Factor XIII in human monocytes and macrophages. J Clin Invest 1985; 76: 528-534
• 7 Adany R, Nemes Z, Thomazy V, Muszbek L. Identification of Factor XIII in macrophages invading lymph nodes with Hodgkin’s disease. In: Haemostasis and Cancer. Muszbek L. (ed) pp 125-134 CRC Press; Boca Raton: 1987
• 8 Adany R, Nemes Z, Muszbek L. Characterization of Factor XIII containing macrophages in lymph nodes with Hodgkin’s disease. Brit J Cancer 1987; 55: 421-426
• 9 Adany R, Kappelmayer J, Muszbek L. Expression of factor XIII subunit A in different types of human macrophages. In: The Biology of Phagocytes in Health and Disease Mauri C, Rizzo SC, Ricevuti G. (eds) Adv Biosci 1987; 66: 323-333
• 10 Murtaugh MP, Arend WP, Davies PJ A. Induction of tissue transglutaminase in human peripheral blood monocytes. J Exp Med 1984; 159: 114-125
• 11 Freundlich B, Avdalovic N. Use of gelatin/plasma coated flasks for isolating human peripheral blood monocytes. J Immunol Methods 1983; 62: 31-37
• 12 Pawlowski NA, Kaplan G, Hamill AL, Cohn ZA, Scott WA. Arachidonic acid metabolism by human monocytes. Studies with platelet-depleted cultures. J Exp Med 1983; 158: 393-412
• 13 Yam LT, Li CY, Crosby WH. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol 1971; 55: 283-290
• 14 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 277: 680-682
• 15 Lorand L, Urayama T, deKiewiet JW C, Nossel HL. Diagnostic and genetic studies on fibrin-stabilizing factor with a new assay based on amine incorporation. J Clin Invest 1969; 48: 1054-1064
• 16 Janossy G, Bofill M, Poulter LW, Rawlings E, Burford GD, Navarete C, Ziegler A, Kelemen E. Separate ontogeny of two macrophage-like accessory cell populations in the human fetus. J Immunol 1986; 136: 4354-4361
• 17 McDonagh J, McDonagh RP, Delage JM W, Wagner RH. Factor XIII in human plasma and platelets. J Clin Invest 1969; 48: 940-946
• 18 Folk JE. Transglutaminases. Ann Rev Biochem 1980; 49: 517-531
• 19 Schroff G, Neumann C, Sorg C. Transglutaminase as a marker of subsets of murine macrophages. Eur J Immunol 1981; 11: 637-642
• 20 Adany R, Kiss A, Muszbek L. Factor XIII: a marker of mono- and megakariocytopoesis. Br J Haematol 1987; 67: 167-72
• 21 Adany R, Glukhova MA, Kabakov AY, Muszbek L. Characterization of human connective tissue cells containing factor XIII subunit a. J Clin Pathol 1987; 41: 49-56
• 22 Kradin RL, Lynch GW, Kurnick JT, Erikson M, Colvin RB, McDonagh J. Factor XIII is synthetized and expressed on the surface of U937 cells and alveolar macrophages. Blood 1987; 69: 778-785
• 23 Weisberg LJ, Shin DT, Concling PR, Shuman MA. Identification of normal human peripheral blood monocytes and liver as sites of synthesis of coagulation Factor XIII a-chainZ. Blood 1987; 70: 579-582
• 24 Shelley WB, Juhlin L. Induction of fibrin thrombi by monocytes. Nature 1977; 270: 343-344
• 25 Lorenzet R, Peri G, Locati D, Allavena P, Colucci M, Semeraro N, Mantovani A, Donati MB. Generation of procoagulant activity by mononuclear phagocytes: a possible mechanism contributing to blood clotting activation within malignant tissues. Blood 1981; 62: 271-273
• 26 Rickies ER, Edwards RL. Activation of blood coagulation in cancer: Trousseau’s syndrome revisited. Blood 1983; 62: 14-31
• 27 Dvorak HF, Senger RD, Dvorak AM. Fibrin as a component of the tumor stroma: origins and biological significance. Cancer Metast Rev 1983; 2: 41-73
• 28 Osterud B, Lindahl U, Seljelid R. Macrophages produce blood coagulation factors. FEBS Letters 1980; 120: 41-43
• 29 Lindahl U, Kolset SO, Bogwald J, Osterud B, Seljelid R. Studies with a luminogenic substrate on blood coagulation factor X/Xa produced by mouse peritoneal macrophages. Biochem J 1982; 206: 231-237
• 30 Chapman HA, Allen LC, Stone OL. Human alveolar macrophages synthesize factor VII in vitro. J Clin Invest 1985; 75: 2030-2037
• 31 Van Dam-Mieras MC E, Muller DA, Van Deijk WA, Hemker HC. Clotting factors secreted by monocytes and macrophages: analytical considerations. Thrombos Res 1985; 37: 9-19
• 32 Duckert F. Documentation of the plasma factor XIII deficiency in man. Ann NY Acad Sci 1972; 202: 190-199
• 33 Mosher DF, Schad PE, Vann JM. Cross-linking of collagen and fibronectin by Factor XHIa. J Biol Chem 1980; 255: 1181-1188
• 34 Leibovich SJ, Ross R. The role of the macrophage in wound repair. Am J Pathol 1975; 78: 71-100
• 35 Muszbek L, Adany R, Lopaciuk S, Kavai M. Possible role of Factor XIII subunit a in phagocytosis by monocytes. Haematol Rev Commun Suppl 1986; 1: 120a Abstr